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Agarose;electrophoresis,Agarose chemical reagent

Location:
Beijing, China ( Mainland)
Status:
New
Sample Availibility:
yes
Payment Terms:
Cash, Bank Transfer, Letter of Credit, T/T, L/C
Categories:
Home > Chemicals > Chemical Reagent

Product Description

In general, Ni2+is the preferred metal ion for purification of histidine-tagged proteins. The IMAC medium Ni Sepharose CL-4B consists of 90 µm beads of highly cross-linked agarose, to which a chelating group has been coupled. This chelating group has then been charged with nickel (Ni2+) ions.Ni Sepharose CL-4B has low Ni2+leakage, high protein-binding capacity, and is compatible with a wide range of additives used in protein purification. Its high flow properties make it excellent for scale-up. The medium is also suitable for gravity flow column purification and multiwell plate screening.

Medium characteristics

Matrix Highly cross-linked spherical agarose, 4%
Dynamic binding capacity* Approx. 40 mg (histidine)6-tagged protein/ml medium
Metal ion capacity

Approx. 15 µmol Ni2+/ml medium

Average particle size

90 µm

Recommended flow rate†

<150 cm/h

Max. operating pressure†

0.1 MPa, 1 bar (when packed in XK columns.

May vary if used in other columns)

Chemical stability‡

Stable in: 0.01 M HCl, 0.1 M NaOH. Tested for 1 week at 40°C.

1 M NaOH, 70% acetic acid. Tested for 12 hours.

2% SDS. Tested for 1 hour.

30% 2-propanol. Tested for 30 min.

pH stability‡

Short term (at least 2 hours) 2–14

Long term (≤1 week) 3–12

Storage 20% ethanol
Storage temperature

4°C to 30°C



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Contact Information

Company:
 Beijing Donglinchangsheng Biotechnology Co., Ltd.
Phone Number:
  86-010-82374028
Fax Number:
 86-010-89756821
 
Beijing Donglinchangsheng Biotechnology Co., Ltd.
 Beijing , China FREE
 

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